The extent of damage on the plasma willbe determined by the release of the beet pigment known as betacyanin. The laboratory experiment will examine the effects of temperature, detergents, and organic solvent on the plasma membrane of the beet, Beta vulgaris. This hypothesis is based on the mere knowledge that environmental and physiological stress denatures protein molecules on the plasma membrane.
The results suggest that betanin, the major betacyanin constituent, may play an important role in the cytotoxicity exhibited by the red beetroot extract. The only conditions that did not disrupt plasma membrane include de-ionized water with a pH value of 7 neutral.
Further studies are needed to evaluate the chemopreventive potentials of the beetroot extract when used alone or in combination with doxorubicin to mitigate the toxic side-effects of the latter.
In contrast, doxorubicin, at the same concentration level, completely inhibited the growth of the PC-3 cells in three days. Betacyanin is contained within the cell membrane and it will be released from the cell when it undergoes physiological and physical stress from environmental factors such as severe temperature, pH, detergents, and organic solvents.
The absorbance value of the betacyanin in the control was 0. The other conditions that disrupted beet cell plasma membrane include the presence of detergents, organic solvents, extreme high temperature, extreme low temperature, low pH and high pH.
Series of experiments will be performed to determine the stability of beet plasma by measuring the quantity of betacyanin produced by the beet cells when subjected to varying conditions: My hypothesis is that extreme temperature ranges, detergents, detergents and organic solvents damage plasma membrane of the beet.
Both doxorubicin and the beetroot extract exhibited a dose-dependent cytotoxic effect in the two cancer cell lines tested.
Although the cytotoxicity of the beetroot extract was significantly lower when compared to doxorubicin, it continued to decrease the growth rate of the PC-3 cells 3.
Similarly, comparative studies in the normal human skin FC and liver HC cell lines showed that the beetroot extract had significantly lower cytotoxic effect than doxorubicin 8. High pH and low pH resulted in absorbance values that were similar to the control. The extreme high temperature and extreme low temperature had the most severe effect on beet plasma membrane integrity followed by detergent, low pH and high pH in that order.
In my experimental prediction, extreme changes in temperature, pH, detergents and organic solvents will damage plasma membrane hence releasing betacyanin.
To further explore this finding, we have compared the cytotoxic effect of the red beetroot extract with anticancer drug, doxorubicin adriamycin in the androgen-independent human prostate cancer cells PC-3 and in the well-established estrogen receptor-positive human breast cancer cells MCF Extreme high temperature, extreme low temperature, detergents and organic solvents result in absorbance vaalues exceeding that of the control in the order of 1.
Hire Writer Betacyanin is a pigment contained in the plasma membrane of the beet. This red colored anticancer antibiotic was selected for comparative cytotoxic study because its chemical structure with a planar configuration of an aromatic chromophore attached to a sugar molecule is remarkably similar to that of betanin, the beetroot extract constituent primarily responsible for its red color.
Explanation of Results During the experiment, it was observed that controls were not affected or were least affected gauging from the least amount of betacyanin that were released from the cell membrane of the beet.This study was aimed to evaluate the total betacyanin content, total phenol and to analyse the antioxidant activity against DPPH radical, superoxide anions, hydroxyl radical, metal chelating, hydrogen.
The results suggest that betanin, the major betacyanin constituent, may play an important role in the cytotoxicity exhibited by the red beetroot extract. Further studies are needed to evaluate the chemopreventive potentials of the beetroot extract when used alone or in combination with doxorubicin to mitigate the toxic side-effects of the latter.
Betacyanin is a pigment contained in the plasma membrane of the beet. Betacyanin is contained within the cell membrane and it will be released from the cell when it undergoes physiological and physical stress from environmental factors such as severe temperature, pH, detergents, and organic solvents.
Red beetroots are rich in betalain pigments, which are divided into betacyanins (predominating in red beetroots) and betaxanthins. The objective of this work was to study efficiency of aqueous extraction of betacyanins from red beets.
A COMPARATIVE STUDY ON THE EXTRACTION OF BETACYANIN IN THE PEEL AND FLESH OF DRAGON FRUIT YUNT KHALIDA BT RIAZUDDIN A project report submitted in partial fulfillment.
Stability Study of Betacyanin Extract from Red Dragon Fruit (Hylocereus Polyrhizus) Peels ☆ Author links open overlay panel Sri Priatni a Aulia Pradita b Show more.Download